Lead alone caused two burst of lipid peroxidation and H2O2 induction at 1 h and 12 h. faba roots depended on its applied chemical form and duration of exposure. The results showed that Pb toxicity to V. Plants were exposed to all treatments for 1, 4, 8, 12, and 24 h in nutrient solution, and contents of H2O2 and thiobarbituric-acid-reactive substances (TBARS) production were analyzed in V. faba seedlings alone or chelated by organic ligands (citric acid and ethylenediaminetetraacetic acid). The current study evaluated the influence of applied form of Pb (metal speciation) on its toxicity to metal sensitive Vicia faba L. These results suggest that under the experimental conditions of our assay, OMWW genotoxicity was associated with gallic acid and oleuropein.Ĭhemical speciation of metals in soil/solution plays an important role in determining their biogeochemical behavior in soil-plant system. Two phenols, gallic acid and oleuropein induced a significant increase in micronucleus frequency in Vicia faba the four other phenols had no significant genotoxic effect. In order to investigate the components involved in this genotoxicity, the six major phenols present in this effluent, oleuropein, gallic acid, 4-hydroxyphenyl acetic acid, caffeic acid, paracoumaric acid and veratric acid, were studied at concentrations corresponding to the genotoxic concentration of the OMWW itself. The results showed that OMWW was genotoxic at 10% dilution. Maleic hydrazide was used as a positive control. Five dilutions of OMWW were tested: 0.1, 1, 5, 10 and 20%. The Vicia faba micronucleus test was used to evaluate the genotoxicity of OMWW and the six major phenolic compounds identified by HPLC in this effluent. The present study concerns the genotoxicity of olive mill waste water (OMWW) generated in mills producing olive oil in Morocco.
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